Supplied as 200 µL purified antibody. Nonphosphorylated p44/42 MAPK (Erk1/2) Control Protein: Bacterially expressed, kinase inactive, Erk2 protein serves as a negative control for Western blotting experiments. p-ERK Antibody (E-4): sc-7383 is recommended for detection of ERK 1 phosphorylated Tyr at the position 204 with a mw of ~44kDa. Synthetic peptide within Human ERK1 + ERK2 aa 196-210 (phospho T202 + Y204) conjugated to keyhole limpet haemocyanin. Correspondingly phosphorylated ERK 2 will also be detected at ~42kDa. Invitrogen Anti-Phospho-GSK3B (Ser9) Polyclonal, Catalog # PA5-104555. This antibody reacts with Human, Mouse, Rat samples. Target information above from: UniProt accession Molecular Weight: ~ 42,44 kDa: Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Belongs to the protein kinase superfamily. Protein A or G purified from hybridoma culture supernatant, Phosphopeptide containing the ERK1 T202/Y204 site. Anti-ERK1 (phospho T202 + Y204) antibody (ab24157) is not available ab24157 is not available and we regret any inconvenience caused. Confocal image showing staining on M phase cells (PMID:26529125). All rights reserved. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Upon delivery aliquot. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221. *Small pack size (SP) is shipped with polar packs. WB analysis using 80031-1-RR. SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway. Molecular Weight: ~ 42,44 kDa: Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Phosphorylates SPZ1 (By similarity). The MAPK/ERK pathway (also known as the Ras-Raf-MEK-ERK pathway) is a chain of proteins in the cell that communicates a signal from a receptor on the surface of the cell to the DNA in the nucleus of the cell.. Molecular Weight: ~ 42,44 kDa: Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Molecular Weight: 327.34. Phosphorylates EIF4EBP1; required for initiation of translation. Detects a band of approximately 44 kDa (predicted molecular weight: 41 kDa). Product Specific Information This antibody detects endogenous protein at a molecular weight of 42 and 44 kDa. Molecular Weight of ERK 1/ERK 2: 44/42 kDa. DATA General Protocols RRID: AB_2840208 p-ERK (E-4) is also recommended for detection of correspondingly phospho-rylated ERK 1 and ERK 2 in additional species, including equine, canine, bovine, porcine and avian. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. This antibody cross-reacts with phosphorylated Erk1 and Erk2. Invitrogen Anti-Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal, Catalog # 44-682G. Get resources and offers direct to your inbox. Full size image. Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal Anti-phospho-Erk1/2 (Thr202/Tyr204 Antibody, Thr185/Tyr187), recombinant clone AW39R detects level of phospho-Erk1/2 (Thr202/Tyr204 & has been published & validated for use in Mplex & WB. DATA Our Cookie Policy explains how you can opt-out of the cookies we use. Molecular weight of MAP kinase (Erk2) protein is 42 kDa. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1. - Find MSDS or SDS, a COA, data sheets and more information. The molecular weight for phospho-MYPT1 (Millipore) was 80 kDa. Store at -20°C long term. This antibody cross-reacts with phosphorylated Erk1 and Erk2. Molecular Weight: ~ 42,44 kDa: ... TFSA increased levels of phospho-ERK and phospho-Akt, enhanced release of BDNF, which were blocked by ERK and PI3K inhibitors, respectively (U0126 and LY294002). Avoid repeated freeze-thaw cycles. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

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